Integrated Human T-Cell Leukemia Virus H Genome in CD8 + T Cells From a Patient With “Atypical ” Hairy Cell Leukemia: Evidence for Distinct

We previously reported isolation of human T-cell leukemia virus II (HTLV-Il) from a second patient (N.R.A.) with atypical hairy cell leukemia. Follow-up analysis of the characteristics of the patient’s HTLV-ll infection over a 2-year period has revealed that the patient had two coexis-tant lymphoproliferative disorders. Oligoclonally integrated HTLV-lI was detected in DNA extracted from the patient’s peripheral blood mononuclear cells on separate occasions greater than 1 year apart. similar to integration of HTLV-l seen in adult T cell leukemia/lymphoma. Although inte-grated provirus was readily detected. no HTIV-Il viral RNA expression was seen in fresh peripheral blood lymphoid cells. Although the patient’s peripheral blood consistently contained a majority of atypical lymphoid cells with a I cell antigenic phenotype. he ultimately developed extensiv